Use of a yeast site-specific recombinase to produce female germline chimeras in Drosophila.
نویسندگان
چکیده
منابع مشابه
Use of a yeast site-specific recombinase to produce female germline chimeras in Drosophila.
We describe an efficient method for generating female germline mosaics by inducing site-specific homologous mitotic recombination with a yeast recombinase (FLP) which is driven by a heat shock promoter. These germline mosaics are produced in flies heterozygous for the agametic, germline-dependent, dominant female sterile (DFS) mutation ovoD1, where only flies possessing germline clones are able...
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ژورنال
عنوان ژورنال: Genetics
سال: 1992
ISSN: 1943-2631
DOI: 10.1093/genetics/131.3.643